Optimizing protein expression in the One-Pot PURE System: Insights into reaction composition and translation efficiency
Zhang Y, Qiu Y, Deveikis M, Martinez ZA, Chou T, Freemont PS, Murray RS
bioRxiv 2024.06.19.599772
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224362 | PT7lacO-His6-AlaRS | Express AlaRS protein for his-tagged purification |
| 224363 | PT7lacO-His6-AsnRS | Express AsnRS protein for his-tagged purification |
| 224364 | PT7lacO-His6-PheRSa-PheRSb | Express PheRS protein for his-tagged purification |
| 224365 | PT7lacO-His6-ThrRS | Express ThrRS protein for his-tagged purification |
| 224366 | PT7lacO-His6-IF1 | Express IF1 protein for his-tagged purification |
| 224367 | PT7lacO-His6-IF2 | Express IF2 protein for his-tagged purification |
| 224368 | PT7lacO-His6-IF3 | Express IF3 protein for his-tagged purification |
| 224369 | PT7lacO-EF-G-His8 | Express EF-G protein for his-tagged purification |
| 224370 | PT7lacO-EF-Tu-His8 | Express EF-Tu protein for his-tagged purification |
| 224371 | PT7lacO-EF-Ts-His6 | Express EF-Ts protein for his-tagged purification |
| 224372 | PT7lacO-RF1-His6 | Express RF1 protein for his-tagged purification |
| 224373 | PT7lacO-RF3-His6 | Express RF3 protein for his-tagged purification |
| 224374 | PT7lacO-RRF-His6 | Express RRF protein for his-tagged purification |
| 224375 | PT7lacO-His6-CKM | Express CKM protein for his-tagged purification |
| 224376 | PT7lacO-His6-NDK | Express NDK protein for his-tagged purification |