At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring.

Jung JK, Rasor BJ, Rybnicky GA, Silverman AD, Standeven J, Kuhn R, Granito T, Ekas HM, Wang BM, Karim AS, Lucks JB, Jewett MC
ACS Synth Biol. 2023 Oct 20;12(10):2909-2921. doi: 10.1021/acssynbio.3c00223. Epub 2023 Sep 12. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
167215	pJBL7044	overexpression of PbrR in E. coli for cell-free lead sensing
167216	pJBL7045	overexpression of CueR in E. coli for cell-free copper sensing
167225	pJBL7056	orthogonal T7 RNAP sfGFP reporter
167230	pJBL7061	cell-free cascaded lead sensor
167231	pJBL7062	cell-free cascaded copper sensor
167244	pJBL7079	cell-free lac sensor with GFP output
167245	pJBL7080	cell-free lac sensor with RFP output
167246	pJBL7081	cell-free lac sensor with Mango output
167247	pJBL7082	cell-free lac sensor with XylE output
167248	pJBL7084	overexpression of LacI
167249	pJBL7085	orthogonal mRFP reporter output for cell-free expression
167250	pJBL7086	orthogonal Mango reporter output for cell-free expression