Zur and zinc increase expression of E. coli ribosomal protein L31 through RNA-mediated repression of the repressor L31p.
Rasmussen RA, Wang S, Camarillo JM, Sosnowski V, Cho BK, Goo YA, Lucks JB, O'Halloran TV
Nucleic Acids Res. 2022 Dec 9;50(22):12739-12753. doi: 10.1093/nar/gkac1086.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185024 | pRAR017-L31-sfGFP | 400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185025 | pRAR039 | 400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185026 | pRAR040 | 400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185027 | pRAR041 | 400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185029 | pRAR051 | 400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185030 | pRAR052 | 400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185031 | pRAR064 | 400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185032 | pRAR074-control-sfGFP | J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185033 | pRAR102 | 400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185034 | pRAR104 | 400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185035 | pRAR105 | 400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185036 | pRAR106 | 400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185037 | pRAR107 | 400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185038 | pRAR109 | 400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185039 | pRAR110 | 400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185040 | pRAR116 | 400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185041 | pRAR120 | J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB |
| 185042 | pRAR089-L36p | J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB |
| 185043 | pRAR090-L36 | J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB |
| 185044 | pRAR091-L31p | J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB |
| 185045 | pRAR092-L31 | J23108(spel), pHP14 stability hairpin, strong RBS, L31 coding region, trrnB |
| 185046 | pRAR101 | J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnB |
| 185047 | pRAR111 | J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnB |
| 185048 | pRAR112 | J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region K2A mutation, trrnB |
| 185049 | pRAR113 | J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnB |
| 185050 | pRAR114 | J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB |
| 185051 | pRAR119 | J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB |