TLNRD1 is a CCM complex component and regulates endothelial barrier integrity.
Ball NJ, Ghimire S, Follain G, Pajari AO, Wurzinger D, Vaitkeviciute M, Cowell AR, Berki B, Ivaska J, Paatero I, Goult BT, Jacquemet G
J Cell Biol. 2024 Sep 2;223(9):e202310030. doi: 10.1083/jcb.202310030. Epub 2024 Jul 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224065 | CCM2(413-438)_pHisSUMO(K0) | Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site |
| 224066 | CCM2(FL)_pET49b | Bacterial expression of full-length CCM2 fused to an N-terminal GST. HRV-3C cleavage site |
| 224067 | TLNRD1(4H)_pEGFP-N3 | Mamalian expression of the 4-helix bundle of TLNRD1 (residues 143-273) fused to a C-terminal EGFP |
| 224068 | TLNRD1(4H)-2E_pET151 | Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site |
| 224069 | TLNRD1(4H)-2T_pET47b | Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site |
| 224070 | TLNRD1(FL)_pEGFP-C1 | Mamalian expression of full-length TLNRD1 fused to an N-terminal EGFP |