TLNRD1 is a CCM complex component and regulates endothelial barrier integrity.
Ball NJ, Ghimire S, Follain G, Pajari AO, Wurzinger D, Vaitkeviciute M, Cowell AR, Berki B, Ivaska J, Paatero I, Goult BT, Jacquemet G
J Cell Biol. 2024 Sep 2;223(9):e202310030. doi: 10.1083/jcb.202310030. Epub 2024 Jul 16.
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Article
Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208861 | TLNRD1_5H_eGFP | Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells. |
| 208876 | CCM2_wt_eGFP | Express eGFP-CCM2 in mammalian cells. |
| 208877 | CCM2_d410-444_eGFP | Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells. |
| 208878 | CCM2_I428S_eGFP | Express eGFP-CCM2 with the I428S mutation in mammalian cells. |
| 208879 | CCM2_I432D_eGFP | Express eGFP-CCM2 with the I432D mutation in mammalian cells. |
| 208880 | CCM2_W421AD422A_eGFP | Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells. |
| 208881 | TLNRD1_35-345_eGFP | Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells. |
| 208883 | TLNRD1_2T_eGFP | Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells. |
| 208884 | TLNRD1_2E_eGFP | Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells. |
| 208886 | mito-CCM2-mScarlet-I | Express mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells. |
| 208887 | mito-PDCD10-mScarlet-I | Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells. |
| 224065 | CCM2(413-438)_pHisSUMO(K0) | Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site |
| 224066 | CCM2(FL)_pET49b | Bacterial expression of full-length CCM2 fused to an N-terminal GST. HRV-3C cleavage site |
| 224067 | TLNRD1(4H)_pEGFP-N3 | Mamalian expression of the 4-helix bundle of TLNRD1 (residues 143-273) fused to a C-terminal EGFP |
| 224068 | TLNRD1(4H)-2E_pET151 | Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site |
| 224069 | TLNRD1(4H)-2T_pET47b | Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site |
| 224070 | TLNRD1(FL)_pEGFP-C1 | Mamalian expression of full-length TLNRD1 fused to an N-terminal EGFP |