4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts
Taschner M, Dickinson JB, Roisné-Hamelin F, Gruber S
bioRxiv 2024.06.17.599261
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223851 | pMulti_A_ccdB | GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance) |
| 223854 | pD(P+T)_T7 | 4G-cloning donor for bacterial promoter and terminator, no tag added |
| 223855 | pD(P+T)_T7N | 4G-cloning donor for bacterial promoter and terminator, N-terminal tag added |
| 223856 | pD(P+T)_T7C | 4G-cloning donor for bacterial promoter and terminator, C-terminal tag added |
| 223857 | pD(P+T)_ph | 4G-cloning donor for insect promoter and terminator, no tag added |
| 223858 | pD(P+T)_phN | 4G-cloning donor for insect promoter and terminator, N-terminal tag added |
| 223859 | pD(P+T)_phC | 4G-cloning donor for insect promoter and terminator, C-terminal tag added |
| 223860 | pD(P+T)_CMV | 4G-cloning donor for mammalian promoter and terminator, no tag added |
| 223861 | pD(P+T)_CMVN | 4G-cloning donor for mammalian promoter and terminator, N-terminal tag added |
| 223862 | pD(P+T)_CMVC | 4G-cloning donor for mammalian promoter and terminator, C-terminal tag added |
| 223863 | pD(Gib)_5'alpha | 4G-cloning donor for 5'-Gibson overhang, alpha-sequence |
| 223864 | pD(Gib)_5'beta | 4G-cloning donor for 5'-Gibson overhang, beta-sequence |
| 223865 | pD(Gib)_5'gamma | 4G-cloning donor for 5'-Gibson overhang, gamma-sequence |
| 223866 | pD(Gib)_5'delta | 4G-cloning donor for 5'-Gibson overhang, delta-sequence |
| 223867 | pD(Gib)_3'beta | 4G-cloning donor for 3'-Gibson overhang, beta-sequence |
| 223868 | pD(Gib)_3'gamma | 4G-cloning donor for 3'-Gibson overhang, gamma-sequence |
| 223869 | pD(Gib)_3'delta | 4G-cloning donor for 3'-Gibson overhang, delta-sequence |
| 223870 | pD(Gib)_3'omega | 4G-cloning donor for 3'-Gibson overhang, omega-sequence |
| 223871 | pD(Nt)_His6 | 4G-cloning donor for N-terminal His6 tag |
| 223872 | pD(Nt)_His6-TEV | 4G-cloning donor for N-terminal His6-TEV tag |
| 223873 | pD(Nt)_His6-3C | 4G-cloning donor for N-terminal His6-3C tag |
| 223874 | pD(Nt)_His6-FLAG3 | 4G-cloning donor for N-terminal His6-FLAG3 tag |
| 223875 | pD(Nt)_His6-FLAG3-TEV | 4G-cloning donor for N-terminal His6-FLAG3-TEV tag |
| 223876 | pD(Nt)_His6-HA2-TEV | 4G-cloning donor for N-terminal His6-HA2-TEV tag |
| 223877 | pD(Nt)_His6-MBP-TEV | 4G-cloning donor for N-terminal His6-MBP-TEV tag |
| 223878 | pD(Nt)_His6-GST-TEV | 4G-cloning donor for N-terminal His6-GST-TEV tag |
| 223879 | pD(Nt)_His6-SUMO | 4G-cloning donor for N-terminal His6-SUMO tag |
| 223880 | pD(Nt)_GFP | 4G-cloning donor for N-terminal GFP tag |
| 223881 | pD(Nt)_GFP-TEV | 4G-cloning donor for N-terminal GFP-TEV tag |
| 223882 | pD(Nt)_GFP-3C | 4G-cloning donor for N-terminal GFP-3C tag |
| 223883 | pD(Nt)_TS-3C | 4G-cloning donor for N-terminal TwinStrep-3C tag |
| 223884 | pD(Nt)_His10-TS-3C | 4G-cloning donor for N-terminal His10-TwinStrep-3C tag |
| 223885 | pD(Nt)_HALO | 4G-cloning donor for N-terminal HALO tag |
| 223886 | pD(Ct)_His8 | 4G-cloning donor for C-terminal His8 tag |
| 223887 | pD(Ct)_3C-His8 | 4G-cloning donor for C-terminal 3C-His8 tag |
| 223888 | pD(Ct)_3C-GFP | 4G-cloning donor for C-terminal 3C-GFP tag |
| 223889 | pD(Ct)_3C-Venus-His8 | 4G-cloning donor for C-terminal 3C-Venus-His8 tag |
| 223890 | pD(Ct)_CPD-His10 | 4G-cloning donor for C-terminal CPD-His10 tag |
| 223891 | pD(Ct)_TS | 4G-cloning donor for C-terminal TwinStrep tag |
| 223892 | pD(Ct)_3C-TS | 4G-cloning donor for C-terminal 3C-TwinStrep tag |
| 223893 | pD(Ct)_3C-TS-His10 | 4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tag |
| 223894 | pD(Ct)_Avi-3C-TS | 4G-cloning donor for C-terminal AviTag-3C-TwinStrep tag |
| 223895 | pD(Ct)_FLAG | 4G-cloning donor for C-terminal FLAG tag |
| 223896 | pD(Ct)_FLAG3 | 4G-cloning donor for C-terminal FLAG3 tag |
| 223897 | pD(Ct)_MYC9 | 4G-cloning donor for C-terminal MYC9 tag |
| 223898 | pD(Ct)_PK6 | 4G-cloning donor for C-terminal PK6 tag |
| 223899 | pD(Ct)_HALO | 4G-cloning donor for C-terminal HALO tag |