Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery
Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Lazarou M, Hummer G, Hurley JH, Martens S
bioRxiv 2024.08.28.609967
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
223723 | pETDuet1_His-TEV-EGFP | Expression of recombinant protein for purification |
223724 | pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa) | Expression of recombinant protein for purification |
223725 | pETDuet1_6xHis-TEV-mCherry-WIPI2d | Expression of recombinant protein for purification |
223726 | pGEX-4T1_GST-LC3A | Expression of recombinant protein for purification |
223727 | pGEX-4T1_GST-LC3C | Expression of recombinant protein for purification |
223728 | pGEX-4T1_GST-GBRP | Expression of recombinant protein for purification |
223729 | pGEX-4T1_GST-GBRPL1 | Expression of recombinant protein for purification |
223730 | pGEX-4T1_GST-GBRPL2 | Expression of recombinant protein for purification |
223731 | pGenLenti V5-NIX | Stable expression of protein in mammalian cell culture |
223732 | pGenLenti V5-BNIP3 | Stable expression of protein in mammalian cell culture |
223733 | pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT) | Expression of recombinant protein for purification |
223734 | pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT) | Expression of recombinant protein for purification |
223735 | pCAG_mCherry-ATG13 (WT) | Expression of recombinant protein for purification |
223736 | pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT) | Expression of recombinant protein for purification |
223737 | pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT) | Expression of recombinant protein for purification |
223738 | pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR) | Expression of recombinant protein for purification |
223739 | pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
223740 | pBIG1a_GST-TEV-CK2alpha_CK2beta | Expression of recombinant protein for purification |
223741 | pBIG1a_GST-TEV-CK2alpha'_CK2beta | Expression of recombinant protein for purification |
223742 | pLIB_GST-TEV-SRC(WT) | Expression of recombinant protein for purification, codon optimized for Insect cells |
223743 | pLIB_GST-TEV-SRC (Y530F) | Expression of recombinant protein for purification |
223744 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST | Expression of recombinant protein for purification |
223745 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST | Expression of recombinant protein for purification |
223746 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A; deltaLIR1) | Expression of recombinant protein for purification |
223747 | pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase | Expression of recombinant protein for purification |
223748 | pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR) | Expression of recombinant protein for purification |
223749 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1) | Expression of recombinant protein for purification |
223750 | pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (Y18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
223751 | pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A) | Expression of recombinant protein for purification |
223752 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2) | Expression of recombinant protein for purification |
223753 | pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2) | Expression of recombinant protein for purification |
223754 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A; deltaLIR1+3) | Expression of recombinant protein for purification |
223755 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A/I307A/V310A; deltaLIR1+4) | Expression of recombinant protein for purification |
223756 | pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4) | Expression of recombinant protein for purification |
223757 | pHAGE-FKBP-mEGFP-WIPI2 | Stable expression and inducible recruitment of protein in mammalian cell culture |
223758 | pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa) | Stable expression and inducible recruitment of protein in mammalian cell culture |
223759 | pCAG_mCherry-ATG13 HORMA (1-191aa) | Expression of recombinant protein for purification |
223760 | pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa) | Expression of recombinant protein for purification |
223761 | pGEX4T1_GST-TEV-EGFP-ATG13_IDR_191-517aa_(M196A_S197A_R199A) | Expression of recombinant protein for purification |
223762 | pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa) | Expression of recombinant protein for purification |
223763 | pETDuet1_6xHis-TEV-mCherry-WIPI3 | Expression of recombinant protein for purification |
223764 | pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST | Expression of recombinant protein for purification |
223765 | pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST | Expression of recombinant protein for purification |
223766 | pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
223767 | pHAGE-FKBP-mEGFP-WIPI1 | Stable expression and inducible recruitment of protein in mammalian cell culture |
223768 | pHAGE-FKBP-mEGFP-WIPI3 | Stable expression and inducible recruitment of protein in mammalian cell culture |
223769 | pHAGE-FKBP-mEGFP-WIPI4 | Stable expression and inducible recruitment of protein in mammalian cell culture |
223770 | pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E) | Stable expression and inducible recruitment of protein in mammalian cell culture |
223771 | pGenlenti_ATG13-WT | Stable expression of protein in mammalian cell culture, rescue experiments |
223772 | pGenlenti_ATG13-delta191-230 | Stable expression of protein in mammalian cell culture, rescue experiments |
223773 | pGenlenti_ATG13-delta191-205 | Stable expression of protein in mammalian cell culture, rescue experiments |
223774 | pGenlenti_ATG13-delta-206-230 | Stable expression of protein in mammalian cell culture, rescue experiments |
223775 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3) | Expression of recombinant protein for purification |
223776 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4) | Expression of recombinant protein for purification |
223777 | pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2) | Expression of recombinant protein for purification |
223778 | pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR) | Expression of recombinant protein for purification |
223779 | pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA) | Expression of recombinant protein for purification |
223780 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A; deltaLIR1+3) | Expression of recombinant protein for purification |
223781 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A/I307A/V310A; deltaLIR1+4) | Expression of recombinant protein for purification |
223782 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2) | Expression of recombinant protein for purification |
223783 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2) | Expression of recombinant protein for purification |
223784 | pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4) | Expression of recombinant protein for purification |
223785 | pGEX4T1_GST-EGFP-ATG13_IDR_delta191-230 (231-517aa) | Expression of recombinant protein for purification |
223786 | pGEX4T1_GST-EGFP-ATG13_IDR_delta191-204 (205-517aa) | Expression of recombinant protein for purification |
223787 | pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa) | Expression of recombinant protein for purification |
223788 | pGenLenti V5-NIX (W36A/L39A; deltaLIR) | Stable expression of protein in mammalian cell culture |
223789 | pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2) | Stable expression of protein in mammalian cell culture |
223790 | pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa) | Expression of recombinant protein for purification |
223791 | pGEX-4T1_GST-EGFP-ATG13_IDR (191-205aa) | Expression of recombinant protein for purification |
223792 | pGEX-4T1_GST-EGFP-ATG13_IDR (191-230aa) | Expression of recombinant protein for purification |
223793 | pGEX-4T1_GST-EGFP-ATG13_IDR (206-230aa) | Expression of recombinant protein for purification |
223794 | pHAGE_FKBP-mEGFP-WIPI2d_P2A-FRB-Fis1 (no mt-mKeima) | Stable expression of protein in mammalian cell culture |
223795 | pHAGE-FRB-Fis1 (no mt-mKeima) | Stable expression of protein in mammalian cell culture |
223796 | pCAG_ATG101 | Expression of recombinant protein for purification (co-expression with ATG13) |
223797 | pCAG_GST-TEV-EGFP-ATG13 | Expression of recombinant protein for purification |
223798 | pCAG_GST-TEV-WIPI1 | Expression of recombinant protein for purification |
223799 | pCAG_WIPI2d-TEV-GST | Expression of recombinant protein for purification |
223800 | pCAG_GST-TEV-WIPI3 | Expression of recombinant protein for purification |
223801 | pCAG_GST-TEV-WIPI4 | Expression of recombinant protein for purification |