FTO mediates LINE1 m(6)A demethylation and chromatin regulation in mESCs and mouse development.
Wei J, Yu X, Yang L, Liu X, Gao B, Huang B, Dou X, Liu J, Zou Z, Cui XL, Zhang LS, Zhao X, Liu Q, He PC, Sepich-Poore C, Zhong N, Liu W, Li Y, Kou X, Zhao Y, Wu Y, Cheng X, Chen C, An Y, Dong X, Wang H, Shu Q, Hao Z, Duan T, He YY, Li X, Gao S, Gao Y, He C
Science. 2022 May 27;376(6596):968-973. doi: 10.1126/science.abe9582. Epub 2022 May 5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223696 | dPspCas13b-wtFTO | Expressing fusion protein of dPspCas13b and wild type human FTO |
| 223697 | dPspCas13b-mutFTO | Expressing fusion protein of dPspCas13b and inactive mutant of human FTO |
| 223698 | pC0043-PspCas13b-gRNA non target | gRNA control for dPspCas13b-FTO |
| 223699 | pC0043-PspCas13b-gRNA LINE1 | gRNA of dPspCas13b-FTO targeting LINE1 |