cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8(+) T cell function and anti-tumor immunity.
Zhao Y, Caron C, Chan YY, Lee CK, Xu X, Zhang J, Masubuchi T, Wu C, Bui JD, Hui E
Immunity. 2023 Jun 13;56(6):1187-1203.e12. doi: 10.1016/j.immuni.2023.04.005. Epub 2023 May 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223596 | pHR-CD28 | Lentiviral expression of human CD28 in mammalian cells |
| 223598 | pHR-Spy-CD86 | Lentiviral expression of human CD86 with SpyTag003 in mammalian cells |
| 223600 | pHR-CLIP-CD28 | Lentiviral expression of human CD28 with CLIP tag in mammalian cells |
| 223601 | pHR-SNAP-CD86iso1 | Lentiviral expression of human CD86 isoform 1 with SNAP tag in mammalian cells |
| 223602 | pHR-SNAP-PDL1 | Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells |
| 223603 | pHR-CD80 | Lentiviral expression of human CD80 in mammalian cells |
| 223604 | pHR-CD86 | Lentiviral expression of human CD86 in mammalian cells |
| 223605 | pHR-CD80-mTFP1 | Lentiviral expression of human CD80 with mTFP1 tag in mammalian cells |
| 223606 | pHR-CD86-mTFP1 | Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells |
| 223607 | pHR-CLIP-CD28(SGGG) | Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells |
| 223608 | pHR-SNAP-CD80(Y65A) | Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells |
| 223609 | pHR-SNAP-CD86(F56A) | Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells |
| 223610 | pHR-CD28-CLIP | Lentiviral expression of human CD28 with CLIP tag in mammalian cells |
| 223612 | pHR-CD80-SNAP | Lentiviral expression of human CD80 with SNAP tag in mammalian cells |
| 223613 | pHR-CD86-SNAP | Lentiviral expression of human CD86 with SNAP tag in mammalian cells |
| 223614 | pHR-SmBiT-CD80 | Lentiviral expression of human CD80 with SmBiT tag in mammalian cells |
| 223616 | pHR-CD28-LgBiT | Lentiviral expression of human CD28 with LgBiT tag in mammalian cells |
| 223618 | pHR-CD86-SmBiT | Lentiviral expression of human CD86 with SmBiT tag in mammalian cells |
| 223619 | pHR-PDL1-LgBiT | Lentiviral expression of human PD-L1 with LgBiT tag in mammalian cells |
| 223620 | FKBP:FRB-FBAR | Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells |
| 223621 | pcDNA-mGFP-Eps15DN | Transient expression of human Eps15 with mGFP tag in mammalian cells |
| 223622 | pHR-Spy-CD28 | Lentiviral expression of human CD28 with SpyTag003 in mammalian cells |
| 223623 | pHR-Spy-CD28(Y191F) | Lentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cells |
| 223624 | pHR-Spy-CD28(CD45TM) | Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells |
| 223625 | pHR-CD28(Y191F) | Lentiviral expression of human CD28 (Y191F) in mammalian cells |
| 223626 | pHR-CD28(CD45TM) | Lentiviral expression of human CD28 (CD45TM) in mammalian cells |
| 223627 | pHR-CD28(Y209F) | Lentiviral expression of human CD28 (Y209F) in mammalian cells |
| 223628 | pHR-CLIP-CD28(Y191F) | Lentiviral expression of human CD28 (Y191F) with CLIP tag in mammalian cells |
| 223629 | pHR-CLIP-CD28(CD45TM) | Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells |
| 223630 | pHR-ALFA-CD28 | Lentiviral expression of human CD28 with ALFA tag in mammalian cells |
| 223632 | pHR-mGFP-SNX9 | Lentiviral expression of human SNX9 with mGFP tag in mammalian cells |
| 223633 | pET28a-SpyCatcher | Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli |
| 223634 | pET28a-LgBiT-SpyCatcher | Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli |
| 223635 | pET28a-SpyCatcher -AVI | Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli |
| 223636 | pET28a-AP-SpyCatcher | Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli |
| 223638 | pHR-CD28-mTFP1 | Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells |
| 223640 | pQCX-CD28 WT | Retroviral expression of mouse CD28 in mammalian cells |
| 223641 | pQCX-CD28 Y189F | Retroviral expression of mouse CD28 (Y189F) in mammalian cells |