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A sensitive biosensor of endogenous Galpha(i) activity enables the accurate characterization of endogenous GPCR agonist responses.

Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M
Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 10.1126/scisignal.adp6457. Epub 2025 Mar 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
223556p3xFLAG-CMV-14-GINIP-NlucPlasmid expressing GINIP-Nluc in mammalian cells
223557p3xFLAG-CMV-14-GINIP-Nluc W139APlasmid expressing GINIP-Nluc W139A in mammalian cells
223558YFP-CAAXPlasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box
223559pLVX-CMV-YFP-CAAX-IRES*-GINIP-NlucLentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor
223560pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139ALentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

Antibodies from Article