A sensitive biosensor of endogenous Galpha(i) activity enables the accurate characterization of endogenous GPCR agonist responses.
Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M
Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 10.1126/scisignal.adp6457. Epub 2025 Mar 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223556 | p3xFLAG-CMV-14-GINIP-Nluc | Plasmid expressing GINIP-Nluc in mammalian cells |
| 223557 | p3xFLAG-CMV-14-GINIP-Nluc W139A | Plasmid expressing GINIP-Nluc W139A in mammalian cells |
| 223558 | YFP-CAAX | Plasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box |
| 223559 | pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc | Alias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor |
| 223560 | pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A | Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor |