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Direct selection of Pichia pastoris expression strains using new G418 resistance vectors.

Lin-Cereghino J, Hashimoto MD, Moy A, Castelo J, Orazem CC, Kuo P, Xiong S, Gandhi V, Hatae CT, Chan A, Lin-Cereghino GP
Yeast. 2008 Apr;25(4):293-9. doi: 10.1002/yea.1587. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
223523pKanBUsed for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker
223524pKanB alphaUsed for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Antibodies from Article