Click strategies for single-molecule protein fluorescence.

Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Plass T, Neal AP, Lemke EA
J Am Chem Soc. 2012 Mar 21;134(11):5187-95. doi: 10.1021/ja210587q. Epub 2012 Mar 5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
223510	pBAD_FLAG-GFP(Y39TAG)-6His	Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39
223511	pEVOL_PylRS(AF)-tRNA	PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon