Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy.
Nikic I, Estrada Girona G, Kang JH, Paci G, Mikhaleva S, Koehler C, Shymanska NV, Ventura Santos C, Spitz D, Lemke EA
Angew Chem Int Ed Engl. 2016 Dec 23;55(52):16172-16176. doi: 10.1002/anie.201608284. Epub 2016 Nov 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223508 | pCMV_NESPylRS(AF)_hU6tRNAPyl | PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon |
| 223509 | pCMV_NESPylRS(WT)_hU6tRNAPyl | PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon |