Native Aminoacyl-tRNA Synthetase/tRNA Pair Drives Highly Efficient Noncanonical Amino Acid Incorporation in Escherichia coli.
Ficaretta ED, Singha Roy SJ, Voss L, Chatterjee A
ACS Chem Biol. 2024 Jun 24. doi: 10.1021/acschembio.4c00221.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218766 | pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr | encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG |
| 218767 | pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr | encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG |
| 218774 | ATMY-C321 | E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR |