Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)
Petruškevičiūtė A, Šimuliūnaitė U, Polanco CM, Rojas B, Kuras S, Valatkaitė-Rakštienė B, Norvilas R, Vijaya AK, Muñoz P, Neniškytė U, Jakubauskas A, Burokas A, Nalvarte I, Inzunza J, Naumovas D, Stoškus M, Griškevičius L, Baltriukienė D, Arias J
Stem Cell Res. 2024 Dec 12:103628. doi: 10.1016/j.scr.2024.103628.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223321 | pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9 | It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid. |
| 223323 | pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9 | Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033). |
| 223328 | pDONOR-MARINA | DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234). |