Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag
Brumbaugh-Reed EH, Aoki K, Toettcher JE
bioRxiv 2024.01.16.575860
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223286 | pHR myc-MBP-SspB-iRFP-NLS | An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system. |
| 223287 | pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE | A complete OptoMBP cassette for dissolution of FUS-CHOP condensates. |
| 223288 | pHR hEF1A-BFP-P2A-rtTA-WPRE | A lentiviral vector expressing BFP and the TetON transcription factor rtTA. |