All-RNA-mediated targeted gene integration in mammalian cells with rationally engineered R2 retrotransposons.
Chen Y, Luo S, Hu Y, Mao B, Wang X, Lu Z, Shan Q, Zhang J, Wang S, Feng G, Wang C, Liang C, Tang N, Niu R, Wang J, Han J, Yang N, Wang H, Zhou Q, Li W
Cell. 2024 Jul 1:S0092-8674(24)00694-9. doi: 10.1016/j.cell.2024.06.020.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223246 | IVT-donor_opt(R2Tg)-GFP | In vitro transcription of the optimized R2Tg RNA donor |
| 223247 | IVT-R2Tg_opt | In vitro transcription of the optimized R2Tg protein |
| 223248 | mCherry-pCAG-donor(R2Tg)-GFP(intron) | Mammalian expression of the R2Tg RNA donor |
| 223249 | mCherry-pCAG-donor_opt(R2Tg)-GFP(intron) | Mammalian expression of the optimized R2Tg RNA donor |
| 223250 | pCAG-R2Tg-3xFlag | Mammalian expression of C-terminally Flag-tagged R2Tg protein |
| 223251 | pCAG-R2Tg_opt-3xFlag | Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein |
| 223252 | pEF1a-donor_opt(R2Tg)-GFP(intron) | Mammalian expression of the optimized R2Tg RNA donor |
| 228504 | mCherry-pCAG-mock | This plasmid can be used for control experiments and can also be used as a plasmid backbone |
| 228505 | pCAG-protein-mock | This plasmid can be used for control experiments and can also be used as a plasmid backbone |