Low copy CRISPR-Cas13d mitigates collateral RNA cleavage.
Hart SK, Wessels HH, Mendez-Mancilla A, Muller S, Drabavicius G, Choi O, Sanjana NE
bioRxiv [Preprint]. 2024 May 14:2024.05.13.594039. doi: 10.1101/2024.05.13.594039.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223175 | pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE | For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa |