A quantitative assay for the efficiency of RNA-guided genome editing in plants.
Bircheneder M, Schreiber T, Tissier A, Parniske M
Plant J. 2024 Sep;119(5):2564-2577. doi: 10.1111/tpj.16931. Epub 2024 Jul 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223004 | pMB20 | Csy4 with 5' and 3' targets (I) and without Cas for Lotus callus assay |
| 223005 | pMB21 | Csy4 with 5' and 3' targets (II) and without Cas for Lotus callus assay |
| 223006 | pMB22 | Control plasmid without Cas and Csy4 for Lotus callus assay |
| 223007 | pMB23 | Csy4 with 5' and 3' targets (I) and without Cas for Nicotiana leaf assay |
| 223008 | pMB24 | Control plasmid without Cas and Csy4 for Nicotiana leaf assay |
| 223009 | pMB26 | Cas12a and Csy4 with 5' target (I) for Nicotiana leaf assay |
| 223010 | pMB27 | Cas12a and Csy4 with 3' target (I) for Nicotiana leaf assay |
| 223011 | pMB28 | Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay |
| 223012 | pMB45 | Cas12a and Csy4 with 5' and 3' targets (I) for Lotus callus assay |
| 223013 | pMB51 | Cas12a and Csy4 with 5' and 3' targets (II) for Lotus callus assay |
| 223014 | pMB61 | Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay |
| 223016 | pMB105 | Cas9 and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay |
| 223017 | pMB109 | Cas9 and Csy4 with 5' and 3' targets (II) for Lotus callus assay |