Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9.
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell. 2024 May 13:S0092-8674(24)00457-4. doi: 10.1016/j.cell.2024.04.031.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222992 | HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1 | Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA |
| 222993 | HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1 | Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA |
| 222994 | HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1 | Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA |