Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9.

Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell. 2024 May 13:S0092-8674(24)00457-4. doi: 10.1016/j.cell.2024.04.031. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
222994	HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1	Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA
222993	HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1	Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA
222992	HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1	Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA