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CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons.

Sirois CL, Guo Y, Li M, Wolkoff NE, Korabelnikov T, Sandoval S, Lee J, Shen M, Contractor A, Sousa AMM, Bhattacharyya A, Zhao X
Cell Rep. 2024 Jun 11;43(6):114330. doi: 10.1016/j.celrep.2024.114330. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222964pLentiCRISPRv1-49535-sgFmr1_CGG5-2Targeting FMR1 5' UTR for CGG deletion
222965pDonor-FMR1-dCGG0To create 0CGG FMR1 cell line
222966pDonor-FMR1-CGG-BsmBI0CGG with BsmBI site for inserting CGG repeats
222967pLV-Syn1-mCherryMake lentivirus to express mCherry in syn1 expressing cells

Antibodies from Article