CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons.
Sirois CL, Guo Y, Li M, Wolkoff NE, Korabelnikov T, Sandoval S, Lee J, Shen M, Contractor A, Sousa AMM, Bhattacharyya A, Zhao X
Cell Rep. 2024 Jun 11;43(6):114330. doi: 10.1016/j.celrep.2024.114330.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222964 | pLentiCRISPRv1-49535-sgFmr1_CGG5-2 | Targeting FMR1 5' UTR for CGG deletion |
| 222965 | pDonor-FMR1-dCGG0 | To create 0CGG FMR1 cell line |
| 222966 | pDonor-FMR1-CGG-BsmBI | 0CGG with BsmBI site for inserting CGG repeats |
| 222967 | pLV-Syn1-mCherry | Make lentivirus to express mCherry in syn1 expressing cells |
| 222968 | p40651-EFSNS-FMR1_e1-24xms2 | MS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequence |
| 222969 | p40651-EFSNS-FMR1_e1ΔCGG-24xms2 | MS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequence |