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Rapid Human Oogonia-like Cell Specification via Combinatorial Transcription Factor-Directed Differentiation

Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
bioRxiv 2022.07.11.499564 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222904pX330_NANOS3_ctermCas9/sgRNA plasmid for targeting NANOS3
222905pHDR_FIGLA-T2A-mGreenLantern-PuroTKHomology directed repair template for knocking in mGreenLantern reporter to FIGLA.
222906pX330_FIGLA_cterm1Cas9/sgRNA plasmid for targeting FIGLA
222907pX330_FIGLA_cterm2Cas9/sgRNA plasmid for targeting FIGLA
222908pX330_FIGLA_cterm3Cas9/sgRNA plasmid for targeting FIGLA
222909pX330_FIGLA_cterm4Cas9/sgRNA plasmid for targeting FIGLA
222910pHDR_NPM2-T2A-mGreenLantern-PuroTKHomology directed repair template for knocking in mGreenLantern reporter to NPM2.
222911pX330_NPM2_cterm1Cas9/sgRNA plasmid for targeting NPM2
222912pX330_NPM2_cterm2Cas9/sgRNA plasmid for targeting NPM2
222913pX330_NPM2_cterm3Cas9/sgRNA plasmid for targeting NPM2
222914pX330_NPM2_cterm4Cas9/sgRNA plasmid for targeting NPM2
222915pHDR2_TFAP2C-T2A-mGreenLantern-HygroHomology directed repair template for knocking in mGreenLantern reporter to TFAP2C.
222921pPB-cT3G-cERH-LHX8PiggyBac transposon plasmid for doxycycline inducible expression of LHX8
222922pPB-cT3G-cERH-SOHLH1PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1
222923pPB-cT3G-cERH-ZNF281PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281
222924pPB-cT3G-cERP2-PRDM1PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1
222925pPB-cT3G-cERP2-SOX17PiggyBac transposon plasmid for doxycycline inducible expression of SOX17
222926pPB-cT3G-cERP2-ZGLP1PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1
222927pPB-cT3G-cERP2-DLX5PiggyBac transposon plasmid for doxycycline inducible expression of DLX5
222928pPB-cT3G-cERP2-HHEXPiggyBac transposon plasmid for doxycycline inducible expression of HHEX
222929pPB-cT3G-cERP2-FIGLAPiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Antibodies from Article