Rapid Human Oogonia-like Cell Specification via Combinatorial Transcription Factor-Directed Differentiation
Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
bioRxiv 2022.07.11.499564
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222903 | pHDR_NANOS3-T2A-mVenus-PuroTK | Homology directed repair template for knocking in mVenus reporter to NANOS3. |
| 222904 | pX330_NANOS3_cterm | Cas9/sgRNA plasmid for targeting NANOS3 |
| 222905 | pHDR_FIGLA-T2A-mGreenLantern-PuroTK | Homology directed repair template for knocking in mGreenLantern reporter to FIGLA. |
| 222906 | pX330_FIGLA_cterm1 | Cas9/sgRNA plasmid for targeting FIGLA |
| 222907 | pX330_FIGLA_cterm2 | Cas9/sgRNA plasmid for targeting FIGLA |
| 222908 | pX330_FIGLA_cterm3 | Cas9/sgRNA plasmid for targeting FIGLA |
| 222909 | pX330_FIGLA_cterm4 | Cas9/sgRNA plasmid for targeting FIGLA |
| 222910 | pHDR_NPM2-T2A-mGreenLantern-PuroTK | Homology directed repair template for knocking in mGreenLantern reporter to NPM2. |
| 222911 | pX330_NPM2_cterm1 | Cas9/sgRNA plasmid for targeting NPM2 |
| 222912 | pX330_NPM2_cterm2 | Cas9/sgRNA plasmid for targeting NPM2 |
| 222913 | pX330_NPM2_cterm3 | Cas9/sgRNA plasmid for targeting NPM2 |
| 222914 | pX330_NPM2_cterm4 | Cas9/sgRNA plasmid for targeting NPM2 |
| 222915 | pHDR2_TFAP2C-T2A-mGreenLantern-Hygro | Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C. |
| 222916 | pX330_TFAP2C_cterm | Cas9/sgRNA plasmid for targeting TFAP2C |
| 222917 | pHDR2_DAZL-T2A-mGreenLantern-PuroTK | Homology directed repair template for knocking in mGreenLantern reporter to DAZL. |
| 222919 | pX330_DAZL_cterm2 | Cas9/sgRNA plasmid for targeting DAZL |
| 222920 | pX330_DAZL_cterm3 | Cas9/sgRNA plasmid for targeting DAZL |
| 222921 | pPB-cT3G-cERH-LHX8 | PiggyBac transposon plasmid for doxycycline inducible expression of LHX8 |
| 222922 | pPB-cT3G-cERH-SOHLH1 | PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1 |
| 222923 | pPB-cT3G-cERH-ZNF281 | PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281 |
| 222924 | pPB-cT3G-cERP2-PRDM1 | PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1 |
| 222925 | pPB-cT3G-cERP2-SOX17 | PiggyBac transposon plasmid for doxycycline inducible expression of SOX17 |
| 222926 | pPB-cT3G-cERP2-ZGLP1 | PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1 |
| 222927 | pPB-cT3G-cERP2-DLX5 | PiggyBac transposon plasmid for doxycycline inducible expression of DLX5 |
| 222928 | pPB-cT3G-cERP2-HHEX | PiggyBac transposon plasmid for doxycycline inducible expression of HHEX |
| 222929 | pPB-cT3G-cERP2-FIGLA | PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA |