Rendering Proteins Fluorescent Inconspicuously: Genetically Encoded 4-Cyanotryptophan Conserves Their Structure and Enables the Detection of Ligand Binding Sites.
Qianzhu H, Abdelkader EH, Welegedara AP, Habel E, Paul N, Frkic RL, Jackson CJ, Huber T, Otting G
Angew Chem Int Ed Engl. 2024 Dec 4:e202421000. doi: 10.1002/anie.202421000.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222871 | pRSF-G1(4CNW)RS | tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon |
| 222872 | pRSF-G1(5CNW)RS | tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon |
| 222873 | pRSF-G1(6CNW)RS | tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon |