Antibodies expand the scope of angiotensin receptor pharmacology.
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol. 2024 May 14. doi: 10.1038/s41589-024-01620-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222846 | pYDS2.0_AT118-H | Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) |
| 222847 | pYDS2.0_AT118-L | Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity |
| 222848 | pET26b_AT118-L-V5-His | E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity |
| 222849 | pET26b_AT118-A-V5-His | E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R) |
| 222850 | pET26b_AT118-H-V5-His | E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) |
| 222851 | pTarget_AT1R-tTA | Mammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activator |
| 222852 | pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG | Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc |
| 222853 | pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A | Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding |
| 222854 | pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG | Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc |
| 222855 | pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A | Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding |
| 222856 | pCDNA3.1_Zeo_TetO_FLAG-AT1R | Mammalian expression plasmid for FLAG-tagged AT1R |