James Hurley Lab: Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex
Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
bioRxiv 2023.06.01.543278
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222821 | pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP | Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His |
| 222822 | His-GST-ATG13 (363-517)-ULK1 (836-1050) | Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 222823 | His-GST-ATG13 (363-517) | Bacteria expression of His-GST -ATG13 C-terminal |
| 222824 | MBP-ATG13 (363-517)-ULK1 (836-1050)-His | Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His |
| 222825 | MBP-ATG13 (450-517)-ULK1 (836-1050)-His | Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His |