Factors and Conditions That Impact Electroporation of Clostridioides difficile Strains.
Bhattacharjee D, Sorg JA
mSphere. 2020 Mar 4;5(2):e00941-19. doi: 10.1128/mSphere.00941-19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132665 | pKM197 | pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant. |