Bridge RNAs direct programmable recombination of target and donor DNA.
Durrant MG, Perry NT, Pai JJ, Jangid AR, Athukoralage JS, Hiraizumi M, McSpedon JP, Pawluk A, Nishimasu H, Konermann S, Hsu PD
Nature. 2024 Jun;630(8018):984-993. doi: 10.1038/s41586-024-07552-4. Epub 2024 Jun 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222722 | pNP555 | sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression) |
| 222723 | pNP561 | Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase |
| 222724 | pNP566 | pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase |
| 222725 | pNP568 | pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis |
| 222726 | pNP1047 | pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis |
| 222727 | pNP1723 | sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression) |
| 222728 | pNP1830 | pNP561 - T5 Target with T7 Inducible IS621 Recombinase |
| 222729 | pNP1950 | J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase |
| 222730 | pNP1954 | pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT) |
| 222732 | pNP2157 | pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase |
| 222733 | pNP2270 | pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase |