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Bridge RNAs direct programmable recombination of target and donor DNA.

Durrant MG, Perry NT, Pai JJ, Jangid AR, Athukoralage JS, Hiraizumi M, McSpedon JP, Pawluk A, Nishimasu H, Konermann S, Hsu PD
Nature. 2024 Jun;630(8018):984-993. doi: 10.1038/s41586-024-07552-4. Epub 2024 Jun 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222722pNP555sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)
222723pNP561Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase
222724pNP566pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase
222725pNP568pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis
222726pNP1047pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis
222727pNP1723sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)
222728pNP1830pNP561 - T5 Target with T7 Inducible IS621 Recombinase
222729pNP1950J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase
222732pNP2157pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase
222733pNP2270pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

Antibodies from Article