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A Bacterial Platform for Studying Ubiquitination Cascades Anchored by SCF-Type E3 Ubiquitin Ligases.

Pu ZX, Wang JL, Li YY, Liang LY, Tan YT, Wang ZH, Li BL, Guo GQ, Wang L, Wu L
Biomolecules. 2024 Sep 25;14(10):1209. doi: 10.3390/biom14101209. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222709pACYCDuet-AtTIR1-Myc-MBP-HAExpression AtTIR1-Myc and MBP-HA in in bacterial cells
222710pACYCDuet-Myc-MBP-AtIAA6-HAExpression MBP-AtIAA6-HA in in bacterial cells
222711pACYCDuet-Myc-MBP-HAExpression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cells
222712pCDFDuet-AtUBA1-SExpression AtUBA1-S in in bacterial cells
222714pCDFDuet-AtUBC8-S-AtUBA1-SExpression AtUBA1-S and AtUBC8-S in in bacterial cells
222715pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-SExpression AtRBX1-T7, AtASK1-T7 and AtCUL1-S in in bacterial cells
222720pRSFDuet-HIS-FLAG-AtUBQ11Expression HIS-FLAG-AtUBQ11 in in bacterial cells

Antibodies from Article