Open-ended molecular recording of sequential cellular events into DNA
Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
bioRxiv 2021.11.05.467507
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226661 | pTBL2142 MTK3-sfGFP | MTK3 part containing the coding sequence of sfGFP |
| 226662 | pTBL3445 MTK3-mTagBFP2 | MTK3 part containing the coding sequence of mTagBFP2 |
| 226663 | pTBL2670 MTK3-PEmax | MTK3 part containing the coding sequence of PEmax |
| 226664 | pTBL3478 PB-PEmax-mTagBFP2 | PiggyBac cargo vector encoding PEmax and mTagBFP2 |
| 226665 | pTBL3346 PB-nicking guide-blast | PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin |
| 226666 | pTBL3241 PB-AtoBpegRNA-mCherry | PiggyBac cargo vector encoding A to B pegRNA marked with mCherry |
| 226667 | pTBL3401 BtoApegRNA-sfGFP | PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP |
| 226668 | pTBL3331 MTK234-TetO-AtoBpegRNA | MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA |
| 226669 | pTBL3428 MTK234-LacO-BtoApegRNA | MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA |
| 226670 | pTBL2620 MTK3-TetR | MTK3 part containing the coding sequence of TetR |
| 226671 | pTBL2621 MTK3-LacI | MTK3 part containing the coding sequence of LacI |
| 226672 | pTBL3481 MTK3-mCherry | MTK3 part containing the coding sequence of mCherry. |
| 226673 | pTBL2368 MTK PB puro backbone | Empty MTK PB cargo backbone marked with puromycin resistance. |
| 226675 | pTBL2367 MTK PB empty backbone with no marker | Empty MTK PB cargo backbone with no marker. |
| 226676 | pTBL3328 PB-sfGFP-WPRE-target | PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site. |