The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation.

Xiao YX, Lee SY, Aguilera-Uribe M, Samson R, Au A, Khanna Y, Liu Z, Cheng R, Aulakh K, Wei J, Farias AG, Reilly T, Birkadze S, Habsid A, Brown KR, Chan K, Mero P, Huang JQ, Billmann M, Rahman M, Myers C, Andrews BJ, Youn JY, Yip CM, Rotin D, Derry WB, Forman-Kay JD, Moses AM, Pritisanac I, Gingras AC, Moffat J
Cell Rep. 2024 Jul 23;43(7):114417. doi: 10.1016/j.celrep.2024.114417. Epub 2024 Jul 8. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
222685	pLJM1-TSC22D2-Cterm-C-FLAG	overexpressing C-terminus truncation of TSC22D2 with C-terminal FLAG
222684	pLJM1-TSC22D2-Cterm-N-FLAG	overexpressing C-terminus truncation of TSC22D2 with N-terminal FLAG
222683	pLJM1-TSC22D2-Nterm-C-FLAG	overexpressing N-terminus truncation of TSC22D2 with C-terminal FLAG
222682	pLJM1-TSC22D2-Nterm-N-FLAG	overexpressing N-terminus truncation of TSC22D2 with N-terminal FLAG
222681	pLJM1-TSC22D2-FL-C-FLAG	overexpressing full length TSC22D2 with C-terminal FLAG
222680	pLJM1-TSC22D2-FL-N-FLAG	overexpressing full length TSC22D2 with N-terminal FLAG
222679	pLJM1-NRBP1-Cterm-C-FLAG	overexpressing C-terminus truncation of NRBP1 with C-terminal FLAG
222678	pLJM1-NRBP1-Cterm-N-FLAG	overexpressing C-terminus truncation of NRBP1 with N-terminal FLAG
222677	pLJM1-NRBP1-Nterm-C-FLAG	overexpressing N-terminus truncation of NRBP1 with C-terminal FLAG
222676	pLJM1-NRBP1-Nterm-N-FLAG	overexpressing N-terminus truncation of NRBP1 with N-terminal FLAG