The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation.
Xiao YX, Lee SY, Aguilera-Uribe M, Samson R, Au A, Khanna Y, Liu Z, Cheng R, Aulakh K, Wei J, Farias AG, Reilly T, Birkadze S, Habsid A, Brown KR, Chan K, Mero P, Huang JQ, Billmann M, Rahman M, Myers C, Andrews BJ, Youn JY, Yip CM, Rotin D, Derry WB, Forman-Kay JD, Moses AM, Pritisanac I, Gingras AC, Moffat J
Cell Rep. 2024 Jul 23;43(7):114417. doi: 10.1016/j.celrep.2024.114417. Epub 2024 Jul 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222667 | pYX-N-Puro-mNeon-TSC22D1 | endogenously tag TSC22D1 with N-terminal mNeon |
| 222669 | pYX-N-Blast-miniTurbo-TSC22D2 | endogenously tag TSC22D2 with N-terminal miniTurbo |
| 222670 | pYX-C-mScarlet-Zeo-TSC22D2 | endogenously tag TSC22D2 with C-terminal mScarlet |
| 222671 | pYX-N-Puro-mNeon-TSC22D4 | endogenously tag TSC22D4 with N-terminal mNeon |
| 222674 | pYX-N-Puro-mNeon-WNK3 | endogenously tag WNK3 with N-terminal mNeon |
| 222675 | pLJM1-NRBP1-FL-N-FLAG | overexpressing full length NRBP1 with N-terminal FLAG |
| 222676 | pLJM1-NRBP1-Nterm-N-FLAG | overexpressing N-terminus truncation of NRBP1 with N-terminal FLAG |
| 222677 | pLJM1-NRBP1-Nterm-C-FLAG | overexpressing N-terminus truncation of NRBP1 with C-terminal FLAG |
| 222678 | pLJM1-NRBP1-Cterm-N-FLAG | overexpressing C-terminus truncation of NRBP1 with N-terminal FLAG |
| 222680 | pLJM1-TSC22D2-FL-N-FLAG | overexpressing full length TSC22D2 with N-terminal FLAG |
| 222681 | pLJM1-TSC22D2-FL-C-FLAG | overexpressing full length TSC22D2 with C-terminal FLAG |
| 222682 | pLJM1-TSC22D2-Nterm-N-FLAG | overexpressing N-terminus truncation of TSC22D2 with N-terminal FLAG |
| 222683 | pLJM1-TSC22D2-Nterm-C-FLAG | overexpressing N-terminus truncation of TSC22D2 with C-terminal FLAG |
| 222684 | pLJM1-TSC22D2-Cterm-N-FLAG | overexpressing C-terminus truncation of TSC22D2 with N-terminal FLAG |
| 222685 | pLJM1-TSC22D2-Cterm-C-FLAG | overexpressing C-terminus truncation of TSC22D2 with C-terminal FLAG |