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Crosstalk between Regnase-1 and -3 shapes mast cell survival and cytokine expression.

Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, Monticelli S
Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 10.26508/lsa.202402784. Print 2024 Aug. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222649pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WTFor in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term
222650pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141NFor in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term
222651pUC57 mini-FLAG-HA-Regnase-3 WTFor in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term
222652pUC57 mini-FLAG-HA-Regnase-3 D252NFor in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term
222653pCDNA3-Regnase-1 WTMammalian expression vector to express mouse Regnase-1 WT
222654pCDNA3-Regnase-1 D141NMammalian expression vector to express mouse Regnase-1 D141N mutant
222660pCDH-Regnase-3 WT-T2A-copGFPLentiviral vector to express mouse Regnase-3 WT
222661pCDH-Regnase-3 D252N-T2A-copGFPLentiviral vector to express mouse Regnase-3 D252N

Antibodies from Article