Crosstalk between Regnase-1 and -3 shapes mast cell survival and cytokine expression.
Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, Monticelli S
Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 10.26508/lsa.202402784. Print 2024 Aug.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222648 | pUC57 mini-ZsGreen-P2A | Vector for cloning gene of interest for in vitro transcription |
| 222649 | pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT | For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term |
| 222650 | pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N | For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term |
| 222651 | pUC57 mini-FLAG-HA-Regnase-3 WT | For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term |
| 222652 | pUC57 mini-FLAG-HA-Regnase-3 D252N | For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term |
| 222653 | pCDNA3-Regnase-1 WT | Mammalian expression vector to express mouse Regnase-1 WT |
| 222654 | pCDNA3-Regnase-1 D141N | Mammalian expression vector to express mouse Regnase-1 D141N mutant |
| 222655 | pCDNA3-Regnase-3 WT | Mammalian expression vector to express mouse Regnase-3 WT |
| 222656 | pCDNA3-FLAG-Regnase-3-HA_full-length | Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term |
| 222657 | pCDNA3-FLAG-Regnase-3_1-547 aa | Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term |
| 222658 | pCDNA3-FLAG-Regnase-3_1-409 aa | Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term |
| 222659 | pCDNA3-FLAG-Regnase-3_1-244 aa | Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term |
| 222660 | pCDH-Regnase-3 WT-T2A-copGFP | Lentiviral vector to express mouse Regnase-3 WT |
| 222661 | pCDH-Regnase-3 D252N-T2A-copGFP | Lentiviral vector to express mouse Regnase-3 D252N |
| 222662 | pmiRGLO-Zc3h12a 3’UTR | Luciferase reporter vector containing mouse Zc3h12a 3'UTR |
| 222664 | pScalps-Puro-FLAG-HA-Regnase-3 D252N | Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term |