Excitable Rho dynamics control cell shape and motility by sequentially activating ERM proteins and actomyosin contractility.
Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada R, Umar M, Mavalwala K, Hayer A
Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. Epub 2024 Sep 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222631 | pCAG-mCherry-FKBP-GEF(ITSN1) | Cdc42 activation using rapamycin |
| 222632 | pCAG-mCherry-FKBP-GAP(ARHGAP29) | Rho inactivation using rapamycin |
| 222633 | pLV-RhoB Sensor-IRES-Blast | Rho reporter |
| 222634 | pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast | Rho reporter, sensor dead |
| 222635 | pLV-mRuby3-MLC-IRES-Neo | Reporter for activated NM2 |
| 222636 | pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo | Ezrin activation reporter |
| 222637 | pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo | Ezrin activation reporter, T567 phosphorylation deficient |
| 222638 | pLV-miRFP680-NES-p2A-dTomato-2xrGBD | Rho reporter |
| 222639 | pLV-mScarlet3-CAAX-IRES-Puro | Live-cell plasma membrane marker |