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Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens.

Zhao Y, Tabet D, Rubio Contreras D, Lao L, Kousholt AN, Weile J, Melo H, Hoeg L, Feng S, Cote AG, Lin ZY, Setiaputra D, Jonkers J, Gingras AC, Gomez Herreros F, Roth FP, Durocher D
Mol Cell. 2023 Aug 3;83(15):2792-2809.e9. doi: 10.1016/j.molcel.2023.06.025. Epub 2023 Jul 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222619pcDNA5-FRT/T0-Flag-BRIP1Mammalian expression vector of flag-tagged BRIP1
222620pcDNA5-FRT/T0-Flag-RECQL5Mammalian expression vector of flag-tagged RECQL5
222621pcDNA5-FRT/T0-EGFP-GNB1LMammalian expression vector of flag-tagged GNB1L
222622pHIV(T9)-DERA-sgresisLentiviral vector that expresses sgRNA resistant DERA in mammalian cells

Antibodies from Article