Characterization of Rationally Designed CRISPR/Cas9-Based DNA Methyltransferases with Distinct Methyltransferase and Gene Silencing Activities in Human Cell Lines and Primary Human T Cells.
Guerra-Resendez RS, Lydon SL, Ma AJ, Bedford GC, Reed DR, Kim S, Teran ER, Nishiguchi T, Escobar M, DiNardo AR, Hilton IB
ACS Synth Biol. 2025 Feb 3. doi: 10.1021/acssynbio.4c00569.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222497 | EFS-SpdCas9-Dnmt3A/3L-WT | Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry |
| 222498 | EFS-SpdCas9-Dnmt3A/3L-V1 | Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry |
| 222499 | EFS-SpdCas9-Dnmt3A/3L-V2 | Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry |
| 222500 | EFS-SpdCas9-Dnmt3A/3L-V3 | Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry |
| 222501 | EFS-SpdCas9-Dnmt3A/3L-catΔ | Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry |
| 222502 | TRE3GV-SpdCas9-Dnmt3A/3L-WT | Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry |
| 222503 | TRE3GV-SpdCas9-Dnmt3A/3L-V1 | Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry |
| 222505 | TRE3GV-SpdCas9-Dnmt3A/3L-V2 | Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry |
| 222506 | TRE3GV-SpdCas9-Dnmt3A/3L-V3 | Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry |
| 222508 | TRE3GV-SpdCas9-Dnmt3A/3L-catΔ | Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry |