Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblasts.
Ilia K, Shakiba N, Bingham T, Jones RD, Kaminski MM, Aravera E, Bruno S, Palacios S, Weiss R, Collins JJ, Del Vecchio D, Schlaeger TM
Sci Adv. 2023 Dec;9(48):eadg8495. doi: 10.1126/sciadv.adg8495. Epub 2023 Nov 29.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
229544 | lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE | Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein |
229545 | lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC | Lentiviral construct to express rtTA and SKM proteins |