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Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblasts.

Ilia K, Shakiba N, Bingham T, Jones RD, Kaminski MM, Aravera E, Bruno S, Palacios S, Weiss R, Collins JJ, Del Vecchio D, Schlaeger TM
Sci Adv. 2023 Dec;9(48):eadg8495. doi: 10.1126/sciadv.adg8495. Epub 2023 Nov 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
229544lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRELentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein
229545lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYCLentiviral construct to express rtTA and SKM proteins

Antibodies from Article