Development of a tightly regulated copper-inducible transient gene expression system in Nicotiana benthamiana incorporating suicide exon and Cre recombinase
Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu CH
bioRxiv 2024.03.23.586378
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222436 | pCHW90004 | Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters. |
| 222437 | pCHW110031 | Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters. |
| 222438 | pCHW110032 | Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters. |
| 222439 | pCHW00011 | Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2. |
| 222440 | pCHW100002 | Level 0, CDS module (AATG-GCTT) containing rice OsL5. |
| 222441 | pCHW110033 | Level 0, CDS module (AATG-GCTT) containing Cre recombinase. |
| 222442 | pCHW100003 | Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM. |
| 222443 | pCHW110034 | Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry. |
| 222444 | pCHW110035 | Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop. |
| 222445 | pCHW00014 | Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS. |
| 222446 | pCHW00015 | Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS. |
| 222447 | pCHW00016 | Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS. |
| 222448 | pCHW00019 | Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS. |
| 222449 | pCHW91003 | Level 1, Position 3 containing 35S::RUBY::OCS. |
| 222450 | pCHW91013 | Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS. |
| 222452 | pCHW111101 | Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS. |
| 222453 | pCHW101008 | Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS. |
| 222454 | pCHW101020 | Level 1, Position 2 containing 35S::CUP2-p65::NOS. |
| 222455 | pCHW91015 | Level 1, Position 2 containing Mas::CUP2-VP16::NOS. |
| 222456 | pCHW111111 | Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT. |
| 222457 | pCHW111112 | Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT. |
| 222458 | pCHW111113 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT. |
| 222459 | pCHW111114 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT. |
| 222460 | pCHW111117 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT. |
| 222461 | pCHW111118 | Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT. |
| 222462 | pCHW91069 | Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT. |
| 222463 | pCHW91070 | Level 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT. |