Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor

Downie L, Ferrandiz N, Megan Jones M, Royle SJ
bioRxiv 2024.05.31.596797 Article

Plasmids from Article

ID	Plasmid	Purpose
222413	LBR-FKBP-GFP	To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
222414	FKBP-GFP-BAF	To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
222415	FKBP-GFP-emerin	To express emerin with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
222416	FKBP-GFP-LAP2beta	To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
222418	SH4-FRB-EBFP2	To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells
222419	FRB-mCherry-EEA1	To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells
222420	FRB-mCherry-PLIN3	To express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cells
222421	mScarletI3-6DG5-MAPPER	To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)
222422	LBR(N547D)-FKBP-GFP	To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
222423	LBR(R583Q)-FKBP-GFP	To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
222425	FKBP-LBR(1-245)-GFP	To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells
222426	mEGFP-PLIN3	To express PLIN3 with mEGFP tag in mammalian cells
222427	GFP-FKBP-LBR	To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells