Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor.
Downie L, Ferrandiz N, Courthold E, Jones M, Royle SJ
PLoS Biol. 2025 Jul 10;23(7):e3003249. doi: 10.1371/journal.pbio.3003249. eCollection 2025 Jul.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222413 | LBR-FKBP-GFP | To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |
| 222414 | FKBP-GFP-BAF | To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |
| 222415 | FKBP-GFP-emerin | To express emerin with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |
| 222416 | FKBP-GFP-LAP2beta | To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |
| 222417 | Stargazin-EBFP2-FRB | To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells |
| 222418 | SH4-FRB-EBFP2 | To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells |
| 222419 | FRB-mCherry-EEA1 | To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells |
| 222420 | FRB-mCherry-PLIN3 | To express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cells |
| 222421 | mScarletI3-6DG5-MAPPER | To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15) |
| 222422 | LBR(N547D)-FKBP-GFP | To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |
| 222423 | LBR(R583Q)-FKBP-GFP | To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |
| 222425 | FKBP-LBR(1-245)-GFP | To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells |
| 222426 | mEGFP-PLIN3 | To express PLIN3 with mEGFP tag in mammalian cells |
| 222427 | GFP-FKBP-LBR | To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells |