Improved constructs for bait RNA display in a bacterial three-hybrid assay
Linh D. Nguyen , Hannah LeBlanc & Katherine E. Berry
Sci Rep 15, 3820 (2025)
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222403 | pLN34 - pBait-5GC[1xMS2]-McaS | arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest. |
| 222404 | pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA | arabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator. |
| 222405 | pSS1 - pBait-5GC[1xMS2]-empty | arabinose-induced synthesis of an RNA containing an MS2 hairpin flanked by a 5GC clamp. Used as a three-hybrid control. For cloning purposes, see plasmid #222403. |
| 222406 | pSS2 - pBait-1xMS2-7GC[empty]-TtrpA | arabinose-induced synthesis of an RNA containing an MS2 hairpin, 7GC clamp and trpA terminator. Used as a three-hybrid control. For cloning purposes, see plasmid #222404. |