Cyanamide-inducible expression of homing nuclease I-SceI for iterative genome engineering and parallel promoter characterisation in Saccharomyces cerevisiae
McDonnell L, Evans S, Lu Z, Suchoronczak M, Leighton J, Ordeniza E, Ritchie B, Valado N, Walsh N, Antoney J, Wang C , Scott C, Speight R, Vickers CE, Peng B
bioRxiv 2024.03.26.586389
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218288 | pITESceIcmd | Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1 |
| 218289 | pJQR3 | Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6 |
| 218290 | pToxEXPr6 | Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3 |
| 218291 | pILGFPEasy9R | Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters. |