Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing.
Pandey S, Gao XD, Krasnow NA, McElroy A, Tao YA, Duby JE, Steinbeck BJ, McCreary J, Pierce SE, Tolar J, Meissner TB, Chaikof EL, Osborn MJ, Liu DR
Nat Biomed Eng. 2024 Jun 10. doi: 10.1038/s41551-024-01227-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222337 | wtBxb1 | Plasmid for expressing wild type Bxb1 in the mammalian cells |
| 222338 | evoBxb1 | Plasmid for expressing evolved Bxb1 in the mammalian cells |
| 222339 | eeBxb1 | Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells |
| 222340 | wtBxb1_IVT | Plasmid for wild type Bxb1 mRNA in vitro transcription template |
| 222341 | evoBxb1_IVT | Plasmid for evolved Bxb1 mRNA in vitro transcription template |
| 222342 | eeBxb1_IVT | Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template |
| 222346 | AAVS1 pegRNA1 with trimmed attP | pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation |
| 222347 | AAVS1 pegRNA2 with trimmed attP | pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation |