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Lysosomal membrane proteins LAMP1 and LIMP2 are segregated in the Golgi apparatus independently of their clathrin adaptor binding motif.

Ecard J, Lian YL, Divoux S, Gouveia Z, Vigne E, Perez F, Boncompain G
Mol Biol Cell. 2024 Mar 1;35(3):ar42. doi: 10.1091/mbc.E23-06-0251. Epub 2024 Jan 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222317Str-KDEL-SBP-EGFP-LAMP1Synchronize the trafficking of LAMP1 from the ER.
222318Str-KDEL_SBP-mCherry-LAMP1Synchronize the trafficking of LAMP1 from the ER.
222319Str-KDEL_SBP-EGFP-LAMP1-Y414ASynchronize the trafficking of LAMP1-Y414A from the ER.
222320Str-KDEL_SBP-mCherry-LAMP1-Y414ASynchronize the trafficking of LAMP1-A17 from the ER.
222321Str-KDEL_SBP-EGFP-LAMP2Synchronize the trafficking of LAMP2 from the ER.
222322Str-KDEL_SBP-EGFP-LAPSynchronize the trafficking of LAP from the ER.
222323Str-Endo-KKXX_SBP-EGFP-LIMP2Synchronize the trafficking of LIMP2 from the ER.
222324Mini-Ii-Str_SBP-EGFP-LIMP2Synchronize the trafficking of LIMP2 from the ER.

Antibodies from Article