Protocol for scarless genome editing of human pluripotent stem cell based on orthogonal selective reporters.

Zhao Y, Pan Z, Hong Z, Sun M, Hong Y, Peng X, Li X, Wang X, Wang K
STAR Protoc. 2024 May 23;5(2):103084. doi: 10.1016/j.xpro.2024.103084. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
210465	SOX17-3'gRNA	gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in
210466	SOX17-NLS-tdTomato-EPG	Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus
210468	INS-3'gRNA	gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in
210469	INS-GFP-CD19-EPT	Donor plasmid for knock-in GFP-CD19 into the human INS locus
215545	B2M-SDMutation-EPG	Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
215546	B2M-SDMutation-EhT	Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
215547	B2M-SDMutation-gRNA	gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus
215548	P2A-NLS-tdtomato-EPG backbone	An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP
215549	P2A-mCherry-EhT backbone	An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato