Strong and tunable anti-CRISPR/Cas activities in plants.
Calvache C, Vazquez-Vilar M, Selma S, Uranga M, Fernandez-Del-Carmen A, Daros JA, Orzaez D
Plant Biotechnol J. 2022 Feb;20(2):399-408. doi: 10.1111/pbi.13723. Epub 2021 Oct 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160605 | pUPD2 SV40-AcrIIA4 (GB3333) | SV40-AcrIIA4 codon optimized for N.benthamiana |
| 160606 | pEGB3 alpha1 35s:PIF6:tNos (GB0170) | TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch |
| 160635 | pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344) | TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator |