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LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.

Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C, Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B
Nucleic Acids Res. 2024 May 29:gkae460. doi: 10.1093/nar/gkae460. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
218274pIRHD-TetR-GAL80-Rho1-GAL4Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2
218275pIRGAL3CIntroduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction
218276pIRHDGAL3CIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
218277pIRGAL4AIntroduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction
218278pIRGAL43CIntroduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
218279pIRHDGAL43CIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction
218280pILGFP4QE2CrimsonIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

Antibodies from Article