LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.
Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C, Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B
Nucleic Acids Res. 2024 May 29:gkae460. doi: 10.1093/nar/gkae460.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218274 | pIRHD-TetR-GAL80-Rho1-GAL4 | Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2 |
| 218275 | pIRGAL3C | Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction |
| 218276 | pIRHDGAL3C | Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction |
| 218277 | pIRGAL4A | Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction |
| 218278 | pIRGAL43C | Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction |
| 218279 | pIRHDGAL43C | Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction |
| 218280 | pILGFP4QE2Crimson | Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter |