The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants.
Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabe-Orts JM, Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M, Quintela JC, Fernandez-Del-Carmen A, Granell A, Orzaez D
Front Plant Sci. 2021 Jul 1;12:689937. doi: 10.3389/fpls.2021.689937. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160561 | pVD1 tRNA-gRNA position E2 (GB2239) | tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA |
| 160562 | pVD1 tRNA-gRNA position E3 (GB2240) | tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter |
| 160563 | pVD1 Multiplexing Edit E4 (GB2241) | tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA. |
| 160564 | pVD1 Multiplexing Edit En-1 (GB2242) | tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter. |
| 160565 | pVD1 tRNA-gRNA E4-En-1 (GB2243) | tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter. |
| 160566 | pVD1 Multiplexing Edit E3-E4-En-1 (GB2244) | GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter. |
| 160567 | pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245) | tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA |
| 160628 | pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234) | Module for the constitutive expression of the nptII, Cas9 and DsRed genes. |
| 160646 | pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235) | Module for the constitutive expression of the nptII, Cas9 and DsRed genes. |