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High Throughput Variant Libraries and Machine Learning Yield Design Rules for Retron Gene Editors.

Crawford KD, Khan AG, Lopez SC, Goodarzi H, Shipman S
bioRxiv [Preprint]. 2024 Jul 9:2024.07.08.602561. doi: 10.1101/2024.07.08.602561. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
222223pAGK43Edits NPAS2 Gene.
222224pKDC.100Acceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.
222225pSCL194Expresses site 1/Cas9/Eco1 RT in HIS3 locus.
222226pSCL195Expresses site 2/Cas9/Eco1 RT in HIS3 locus.
222227pSCL368Expresses site 3/Cas9/Eco1 RT in HIS3 locus.
222228pKDC.154Acceptor plasmid for human retron-Eco1 variants.

Antibodies from Article