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Identification of New In Vivo TonB-FepA Rendezvous Sites

Postle K, Kho K, Gresock M, Ghosh J, Larsen R
bioRxiv 2021.12.08.471779 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167593pKP372Express TonB frameshift of amphipathic helix
167594pKP476Express TonB ∆(199-216)
167615pKP1362Express TonB C18G.
167616pKP1427Express TonB C18G, Q160C.
167617pKP1708Express TonB C18G, H20A, Q160C.
167618pKP1676Express TonB C18G, M201C.
167619pKP1720Express TonB C18G, H20A, M201C.
167620pKP1867Express TonB C18G, R204C.
167621pKP1868Express TonB C18G, H20A, R204C.
167622pKP1683Express TonB C18G, V206C.
167623pKP1722Express TonB C18G, H20A, V206C.
167624pKP1646Express TonB C18G, N208C.
167625pKP1692Express TonB C18G, H20A, N208C.
167626pKP1647Express TonB C18G, A209C.
167627pKP2299Express TonB C18G, H20A, A209C.
167628pKP1624Express TonB C18G, R212C.
167629pKP1861Express TonB C18G, H20A, R212C.
167630pKP1684Express TonB C18G, R214C.
167631pKP1723Express TonB C18G, H20A, R214C.
167632pKP2303Express TonB C18G, Q160C, F202A.
167633pKP2304Express TonB C18G, Q160C, F202A, W213A.
167634pKP1410Express FepA D12C . Please note that mutation numbers are not based on start codon of insert.
167636pKP718Express FepA T13C . Please note that mutation numbers are not based on start codon of insert.
167638pKP1411Express FepA I14C . Please note that mutation numbers are not based on start codon of insert.
167639pKP1383Express FepA V15C . Please note that mutation numbers are not based on start codon of insert.
167641pKP1384Express FepA V16C . Please note that mutation numbers are not based on start codon of insert.
167643pKP1416Express FepA T17C . Please note that mutation numbers are not based on start codon of insert.
167645pKP1583Express FepA D34C . Please note that mutation numbers are not based on start codon of insert.
167646pKP1626Express FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.
167647pKP1382Express FepA G54C . Please note that mutation numbers are not based on start codon of insert.
167648pKP1627Express FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.

Antibodies from Article