Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440.
Bleem AC, Kuatsjah E, Johnsen J, Mohamed ET, Alexander WG, Kellermyer ZA, Carroll AL, Rossi R, Schlander IB, Peabody V GL, Guss AM, Feist AM, Beckham GT
Metab Eng. 2024 Jul;84:145-157. doi: 10.1016/j.ymben.2024.06.009. Epub 2024 Jun 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222207 | pACB108 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag |
| 222208 | pACB109 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag |
| 222209 | pACB110 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag |
| 222210 | pACB111 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag |
| 222211 | pACB112 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag |
| 222212 | pACB113 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag |
| 222213 | pACB120 | For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag |
| 222214 | pACB143 | For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag |
| 222220 | pSN95 | For recombinant expression of vanA (PP_3736 from Pseudomonas putida) with a C-terminal His tag |