Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop new strains
Pennetti VJ, LaFayette PR, Parrott WA
bioRxiv 2024.08.04.606528
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222076 | pVP073 | eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites. |
| 222077 | pVP074 | eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites. |
| 222078 | pVP075 | AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites. |
| 222079 | pVP141 | Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion. |
| 222080 | pVP143 | Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction. |
| 222081 | pVP144 | Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction. |