Chiaki Murakami Lab: PHOSPHO2 is PA phosphatase and PHOSPHO1 is PLC
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222014 | pGEX-N-TEV-Twin-Strep | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli |
| 222015 | pCAGGS-PHOSPHO1-TEV-Twin-Strep | Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell |
| 222016 | pCAGGS-PHOSPHO2-TEV-Twin-Strep | Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell |
| 222017 | pGEX-PHOSPHO2-TEV-Twin-Strep | Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli |
| 223720 | pCAGGS-Twin-strep-TEV-DGKδ2 | Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell |
| 223721 | pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD | Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell |
| 224896 | pmCherry-C1-PHOSPHO1 | Express N-terminal mCherry fused to human PHOSPHO1 in mammalian cells |
| 224897 | pAcGFP-C1-DGKd2 | Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells |