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A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins.

Wang J, Choi JM, Holehouse AS, Lee HO, Zhang X, Jahnel M, Maharana S, Lemaitre R, Pozniakovsky A, Drechsel D, Poser I, Pappu RV, Alberti S, Hyman AA
Cell. 2018 Jul 26;174(3):688-699.e16. doi: 10.1016/j.cell.2018.06.006. Epub 2018 Jun 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
221885TH0901-pOCC177-FUS-wt_opt(Nhe)Shuttle vector for baculovirus production, using FlashBac bacmid
221886TH0994-pOCC177-Fus-wt_(1-211)Shuttle vector for baculovirus production, using FlashBac bacmid
221887TH1148-pOCC177-FUS(Nhe)-PLD_YtoFShuttle vector for baculovirus production, using FlashBac bacmid
221888TH1149-pOCC177-FUS(Nhe)-RGG_RtoKShuttle vector for baculovirus production, using FlashBac bacmid
221890TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_optShuttle vector for baculovirus production, using FlashBac bacmid
221891TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAPShuttle vector for baculovirus production, using FlashBac bacmid
221892TH1204-pOCC120-FUS_opt(NheI)Shuttle vector for baculovirus production, using FlashBac bacmid
221893TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFPShuttle vector for baculovirus production, using FlashBac bacmid
221894TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFPShuttle vector for baculovirus production, using FlashBac bacmid
221895TH1263-018_Jie_SFPQ_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFPShuttle vector for baculovirus production, using FlashBac bacmid
221896TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAPShuttle vector for baculovirus production, using FlashBac bacmid

Antibodies from Article